Angelo Agossou Yao (AG Weber)

Subcellular localization and Role of aminotransferases  in C2 photosynthesis metabolite flux

Aminotransferases bridge biosynthetic reactions involving nitrogen (e.g., amino acids, nucleic acids, and chlorophyll) with photosynthesis. Using cofactors, reducing equivalents, and carbon skeletons generated during photosynthesis aminotransferases control the entrance port to primary and secondary nitrogen assimilation and photorespiration generated nitrogen re-assimilation. Thus, in all plant, they play a crucial role in metabolism. Depending on the subcellular localization their catalytic activity and substrate requirement could be considerably different. Though there is no subcellular localization evidence of cytosolic alanine aminotransferase , an accumulation of amino acid in the cell cytosol in many metabolomic data has been attributed to gamma-aminobutyric acid (GABA) activity, which is assumed to convert alanine to pyruvate in arabidopsis during the recovery from hypoxia. Therefore, allowing the plant to bypass energy starvation during recovery phase. My project goals are:

i) Use GFP fusion to localize AlaAT1 in the subcellular compartment of C3, C3-C4 and C4 dicot and C4 monocot plant.

ii) Perform a subcellular knock out of AlaAT1 to access its regulation.

iii) Analyse the metabolite flux balance induced by the C2 cycle and it correlation to the aminotransferase activity on the C3, C3-C4 and C4 flaveria background

Starting date: 15.10.2013 / PhD student

Thesis committee members: Andreas Weber

Mini Academic CV:

  • 2008:
    • Engineer degree (MSc) in Agricultural Science at the University of Abomey Calavi (Benin)
      Thesis: Biochemical and microbiological characterization of fermentation process in Dicentrarchus labrax.
  •  2011:
    • Master of Science (MSc) in functional Plant Science at the University of Rostock.
      Thesis: Accessement of genetic diversity of Calotropis procera in west Africa region (Benin)
Photo of Dagbegnon Angelo Romeo  Agossou Yao

Dagbegnon Angelo Romeo Agossou Yao M. Sc.

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